|Application ||WB, E|
|Other Accession||Q8R060, NP_060445.3|
|Calculated MW||67214 Da|
|Antigen Region||482-508 aa|
|Other Names||Protein zwilch homolog, hZwilch, ZWILCH|
|Target/Specificity||This ZWILCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human ZWILCH.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZWILCH Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).|
|Cellular Location||Chromosome, centromere, kinetochore|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.
Brendle, A., et al. Eur. J. Cancer 45(3):435-442(2009)
Kops, G.J., et al. J. Cell Biol. 169(1):49-60(2005)
Williams, B.C., et al. Mol. Biol. Cell 14(4):1379-1391(2003)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.