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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   ZWILCH Antibody (C-term)   

ZWILCH Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ZWILCH Antibody (C-term) AP18532b
    ZWILCH Antibody (C-term) (Cat. #AP18532b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).
  • WB - ZWILCH Antibody (C-term) AP18532b
    ZWILCH Antibody (C-term) (Cat. #AP18532b) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H900
Other Accession Q8R060, NP_060445.3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67214 Da
Antigen Region 482-508 aa
Additional Information
Gene ID 55055
Other Names Protein zwilch homolog, hZwilch, ZWILCH
Target/Specificity This ZWILCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human ZWILCH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZWILCH Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZWILCH
Function Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).
Cellular Location Chromosome, centromere, kinetochore
Research Areas
Citations (0)

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Background

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.

References

Brendle, A., et al. Eur. J. Cancer 45(3):435-442(2009)
Kops, G.J., et al. J. Cell Biol. 169(1):49-60(2005)
Williams, B.C., et al. Mol. Biol. Cell 14(4):1379-1391(2003)

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$ 295.00
$ 99.00
Cat# AP18532b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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