- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
ACER1 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP18654b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP18654b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ACER1 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q8TDN7 |
| Other Accession | NP_597999.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 31095 Da |
ACER1 Antibody (C-term) - Additional info | |
| Gene ID 125981 | |
| Other Names ACER1; ASAH3; Alkaline ceramidase 1; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3 | |
| Target/Specificity This ACER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from the C-terminal region of human ACER1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACER1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACER1 Antibody (C-term) - Protein Information | |
| Name ACER1 | |
| Synonyms ASAH3 | |
| Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids (By similarity) | |
| Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity) | |
| Tissue Location Mainly expressed in epidermis. | |
ACER1 Antibody (C-term) - Related products
ACER1 Antibody (C-term) - Application data
-
ACER1 Antibody (C-term) (Cat. #AP18654b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ACER1 antibody detected the ACER1 protein (arrow).
ACER1 Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).
REFERENCES
Sun, W., et al. J. Invest. Dermatol. 128(2):389-397(2008) Toulza, E., et al. Genome Biol. 8 (6), R107 (2007) : Houben, E., et al. J. Lipid Res. 47(5):1063-1070(2006) Mao, C., et al. J. Biol. Chem. 278(33):31184-31191(2003) Ito, M., et al. Tanpakushitsu Kakusan Koso 47 (4 SUPPL), 455-462 (2002) :
