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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   API5 Antibody (C-term)   

API5 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - API5 Antibody (C-term) AP18825b
    API5 Antibody (C-term)(Cat. #AP18825b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the API5 antibody detected the API5 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BZZ5
Other Accession NP_006586.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59005 Da
Antigen Region 482-510 aa
Additional Information
Gene ID 8539
Other Names Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL, API5
Target/Specificity This API5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-510 amino acids from the C-terminal region of human API5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPI5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name API5
Function Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic
Tissue Location Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
Research Areas
Citations (0)

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Background

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

References

Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :
Ren, K., et al. Pathol. Oncol. Res. 16(2):229-237(2010)
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Rigou, P., et al. EMBO J. 28(11):1576-1588(2009)
Wang, Y., et al. J. Biol. Chem. 283(19):13205-13215(2008)

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$ 295.00
$ 99.00
Cat# AP18825b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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