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G2E3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - G2E3 Antibody (Center) AP18914C
    G2E3 Antibody (Center) (Cat. #AP18914c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the G2E3 antibody detected the G2E3 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7L622
Other Accession Q4R9C4, NP_060239.2
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 80504 Da
Antigen Region 226-252 aa
Additional Information
Gene ID 55632
Other Names G2/M phase-specific E3 ubiquitin-protein ligase, 632-, G2E3, KIAA1333
Target/Specificity This G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsG2E3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name G2E3
Synonyms KIAA1333
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
Cellular Location Nucleus, nucleolus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In the nucleus, delocalizes from the nucleolus to the nucleoplasm in response to DNA damage
Tissue Location Predominantly expressed in brain, liver, kidney, testes and ovary.
Research Areas
Citations (0)

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Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.

References

Brooks, W.S., et al. J. Biol. Chem. 283(32):22304-22315(2008)
Brooks, W.S., et al. Exp. Cell Res. 313(4):665-676(2007)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Crawford, D.F., et al. J. Biol. Chem. 276(40):37166-37177(2001)

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$ 295.00
$ 99.00
Cat# AP18914C
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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