|Application ||WB, E|
|Other Accession||Q4R9C4, NP_060239.2|
|Calculated MW||80504 Da|
|Antigen Region||226-252 aa|
|Other Names||G2/M phase-specific E3 ubiquitin-protein ligase, 632-, G2E3, KIAA1333|
|Target/Specificity||This G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||G2E3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.|
|Cellular Location||Nucleus, nucleolus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In the nucleus, delocalizes from the nucleolus to the nucleoplasm in response to DNA damage|
|Tissue Location||Predominantly expressed in brain, liver, kidney, testes and ovary.|
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Provided below are standard protocols that you may find useful for product applications.
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
Brooks, W.S., et al. J. Biol. Chem. 283(32):22304-22315(2008)
Brooks, W.S., et al. Exp. Cell Res. 313(4):665-676(2007)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Crawford, D.F., et al. J. Biol. Chem. 276(40):37166-37177(2001)
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