|Application ||WB, E|
|Calculated MW||43250 Da|
|Antigen Region||1-30 aa|
|Other Names||Ras-related GTP-binding protein B, Rag B, RagB, RRAGB (HGNC:19901)|
|Target/Specificity||This RRAGB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RRAGB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RRAGB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway.|
|Cellular Location||Cytoplasm. Lysosome|
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Provided below are standard protocols that you may find useful for product applications.
Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Tomarev, S.I., et al. Invest. Ophthalmol. Vis. Sci. 44(6):2588-2596(2003)
Sekiguchi, T., et al. J. Biol. Chem. 276(10):7246-7257(2001)
Hirose, E., et al. J. Cell. Sci. 111 (PT 1), 11-21 (1998) :
Schurmann, A., et al. J. Biol. Chem. 270(48):28982-28988(1995)
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