|Application ||WB, E|
|Calculated MW||43250 Da|
|Antigen Region||1-30 aa|
|Other Names||Ras-related GTP-binding protein B, Rag B, RagB, RRAGB (HGNC:19901)|
|Target/Specificity||This RRAGB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RRAGB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RRAGB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway.|
|Cellular Location||Cytoplasm. Lysosome|
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Provided below are standard protocols that you may find useful for product applications.
Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Tomarev, S.I., et al. Invest. Ophthalmol. Vis. Sci. 44(6):2588-2596(2003)
Sekiguchi, T., et al. J. Biol. Chem. 276(10):7246-7257(2001)
Hirose, E., et al. J. Cell. Sci. 111 (PT 1), 11-21 (1998) :
Schurmann, A., et al. J. Biol. Chem. 270(48):28982-28988(1995)
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