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ATPIF1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ATPIF1 Antibody (N-term) AP18944a
    ATPIF1 Antibody (N-term) (Cat. #AP18944a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ATPIF1 antibody detected the ATPIF1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UII2
Other Accession NP_057395.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 12249 Da
Antigen Region 8-35 aa
Additional Information
Gene ID 93974
Other Names ATPase inhibitor, mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1, ATPI
Target/Specificity This ATPIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of human ATPIF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATPIF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP5IF1 (HGNC:871)
Synonyms ATPI, ATPIF1
Function Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Cellular Location Mitochondrion.
Research Areas
Citations (0)
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Background

This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].

References

Sanchez-Cenizo, L., et al. J. Biol. Chem. 285(33):25308-25313(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Li, N., et al. Int. J. Neurosci. 120(3):217-221(2010)
Contessi, S., et al. J. Bioenerg. Biomembr. 39(4):291-300(2007)
Ma, J., et al. Atherosclerosis 191(1):63-72(2007)

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$ 182.50
$ 70.00
Cat# AP18944a
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