|Application ||WB, E|
|Other Accession||Q9ERZ8, Q9EPK8, NP_067638.3|
|Calculated MW||98281 Da|
|Antigen Region||160-189 aa|
|Other Names||Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Transient receptor potential protein 12, TRP12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, VRL-2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, TRPV4, VRL2, VROAC|
|Target/Specificity||This TRPV4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human TRPV4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRPV4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Cell junction, adherens junction. Note=Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum Isoform 5: Cell membrane.|
|Tissue Location||Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene.
Shukla, A.K., et al. J. Biol. Chem. 285(39):30115-30125(2010)
Cantero-Recasens, G., et al. J. Biol. Chem. 285(36):27532-27535(2010)
Loukin, S., et al. J. Biol. Chem. 285(35):27176-27181(2010)
Nishimura, G., et al. Am. J. Med. Genet. A 152A (6), 1443-1449 (2010) :
Zimon, M., et al. Brain 133 (PT 6), 1798-1809 (2010) :
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