|Application ||WB, E|
|Other Accession||P63322, P63321, NP_005393.2|
|Calculated MW||23567 Da|
|Antigen Region||158-186 aa|
|Other Names||Ras-related protein Ral-A, RALA, RAL|
|Target/Specificity||This RALA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human RALA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RALA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.|
|Cellular Location||Cell surface. Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody. Note=Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA colocalizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody|
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Provided below are standard protocols that you may find useful for product applications.
The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.
Nichols, C.D., et al. Curr. Biol. 20(14):1316-1320(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Godin, C.M., et al. Mol. Pharmacol. 77(3):388-395(2010)
Lim, K.H., et al. Mol. Cell. Biol. 30(2):508-523(2010)
Wang, K., et al. Int J Immunopathol Pharmacol 22(3):735-743(2009)
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