|Application ||WB, E|
|Other Accession||O35049, Q9JJY3, NP_061137.1|
|Calculated MW||71081 Da|
|Antigen Region||137-165 aa|
|Other Names||Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, nSMase-2, nSMase2, Neutral sphingomyelinase II, SMPD3|
|Target/Specificity||This SMPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the N-terminal region of human SMPD3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMPD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.|
|Cellular Location||Golgi apparatus membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Note=May localize to detergent- resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons (PubMed:10823942). Localizes to plasma membrane in confluent contact-inhaibited cells (PubMed:15051724)|
|Tissue Location||Predominantly expressed in brain.|
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Provided below are standard protocols that you may find useful for product applications.
SMPD3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Filosto, S., et al. J. Biol. Chem. 285(14):10213-10222(2010)
Maupas-Schwalm, F., et al. Cell. Signal. 21(12):1925-1934(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Ito, H., et al. Biochim. Biophys. Acta 1789 (11-12), 681-690 (2009) :
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