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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MSX1 Antibody (Center)   

MSX1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MSX1 Antibody (Center) AP19261C
    Anti-MSX1 Antibody (Center)at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - MSX1 Antibody (Center) AP19261C
    Fluorescent image of U251 cell stained with MSX1 Antibody (Center)(Cat#AP19261c).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).MSX1 immunoreactivity is localized to Nucleus significantly.
  • WB - MSX1 Antibody (Center) AP19261C
    MSX1 Antibody (Center)(Cat. #AP19261c) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).
  • WB - MSX1 Antibody (Center) AP19261C
    MSX1 Antibody (Center) (Cat. #AP19261c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).
  • IF - MSX1 Antibody (Center) AP19261C
    Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19261c MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28360
Other Accession O02786, NP_002439.2
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31496 Da
Antigen Region 111-138 aa
Additional Information
Gene ID 4487
Other Names Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein, MSX1, HOX7
Target/Specificity This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMSX1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MSX1
Synonyms HOX7
Function Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity.
Cellular Location Nucleus.
Tissue Location Expressed in the developing nail bed mesenchyme.
Research Areas
Citations (0)

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This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia.


References for protein:
1.Sliwinski, T., et al. Cancer Epidemiol 34(5):652-655(2010)
2.Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)
3.Suazo, J., et al. Am. J. Med. Genet. A 152A (8), 2011-2016 (2010) :
4.Doi, T., et al. J. Pediatr. Surg. 45(6):1187-1191(2010)
5.Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].

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$ 295.00
$ 99.00
Cat# AP19261C
(40 western blots)
Availability: In Stock
Bulk Size
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