|Application ||WB, E|
|Other Accession||Q80WV3, NP_004258.2|
|Calculated MW||57857 Da|
|Antigen Region||493-522 aa|
|Other Names||Carbohydrate sulfotransferase 2, 282-, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, GST-2, N-acetylglucosamine 6-O-sulfotransferase 1, GlcNAc6ST-1, Gn6ST-1, CHST2, GN6ST|
|Target/Specificity||This CHST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 493-522 amino acids from the C-terminal region of human CHST2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CHST2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein|
|Tissue Location||Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.|
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Provided below are standard protocols that you may find useful for product applications.
N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Desko, M.M., et al. Glycobiology 19(10):1068-1077(2009)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
Kanoh, A., et al. Glycoconj. J. 23 (5-6), 453-460 (2006) :
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