|Application ||WB, E|
|Calculated MW||40748 Da|
|Antigen Region||218-244 aa|
|Other Names||Septin-12, SEPT12|
|Target/Specificity||This SEPT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-244 amino acids from the Central region of human SEPT12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SEPT12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Filament-forming cytoskeletal GTPase (By similarity). Involved in spermatogenesis. Involved in the morphogenesis of sperm heads and the elongation of sperm tails probably implicating the association with alpha- and beta-tubulins (PubMed:24213608). Forms a filamentous structure with SEPT7, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). May play a role in cytokinesis (Potential).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Nucleus. Cell projection, cilium, flagellum. Note=At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis. Found in the sperm annulus. Colocalized with SPAG4 at the nuclear periphery in round spermatids, at sperm neck in elongated spermatids and at midpiece regions in ejaculated spermatozoa.|
|Tissue Location||Widely expressed. Expressed in lymph node.|
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Provided below are standard protocols that you may find useful for product applications.
Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia (Hall et al., 2005 [PubMed 15915442]).
Lin, Y.H., et al. Am. J. Pathol. 174(5):1857-1868(2009)
Ding, X., et al. Mol. Cells 25(3):385-389(2008)
Ding, X., et al. J. Biochem. Mol. Biol. 40(6):973-978(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hall, P.A., et al. J. Pathol. 206(3):269-278(2005)
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