|Application ||WB, E|
|Calculated MW||107496 Da|
|Antigen Region||402-429 aa|
|Other Names||SH3 domain-binding protein 4, EH-binding protein 10, Transferrin receptor-trafficking protein, SH3BP4, BOG25, EHB10, TTP|
|Target/Specificity||This SH3BP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-429 amino acids from the Central region of human SH3BP4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SH3BP4 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BOG25, EHB10, TTP|
|Function||May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin- mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy.|
|Cellular Location||Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus Note=Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus|
|Tissue Location||Expressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of a specific protein receptor.
Sebastiani, P., et al. Science (2010) In press :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Thalappilly, S., et al. Proteomics 8(15):3071-3081(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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