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RECQL5 Antibody(Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O94762 |
| Other Accession | NP_001003715.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 108858 Da |
| Antigen Region | 662-691 aa |
| Gene ID | 9400 |
|---|---|
| Other Names | ATP-dependent DNA helicase Q5, DNA helicase, RecQ-like type 5, RecQ5, RecQ protein-like 5, RECQL5, RECQ5 |
| Target/Specificity | This RECQL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-691 amino acids from the Central region of human RECQL5. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RECQL5 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RECQL5 |
|---|---|
| Synonyms | RECQ5 {ECO:0000303|PubMed:9878247} |
| Function | DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052, PubMed:28100692). Probably unwinds DNA in a 3'-5' direction (PubMed:28100692) (Probable). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription- associated genomic instability (PubMed:20231364). Associates also with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81- EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed:28100692). |
| Cellular Location | [Isoform Beta]: Nucleus, nucleoplasm. Nucleus Note=Recruited to sites of DNA damage, such as single-strand breaks and inter-strand cross-links, and at stalled replication forks. Re- localizes from the nucleolus into the nucleus after replicative stress and significantly associates with WRN during S-phase (PubMed:23180761) [Isoform Gamma]: Cytoplasm |
| Tissue Location | Ubiquitous.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
RECQL5 may have an important role in DNA metabolism.
References
Schwendener, S., et al. J. Biol. Chem. 285(21):15739-15745(2010)
Islam, M.N., et al. Mol. Cell. Biol. 30(10):2460-2472(2010)
Speina, E., et al. Nucleic Acids Res. 38(9):2904-2916(2010)
Aygun, O., et al. J. Biol. Chem. 284(35):23197-23203(2009)
Aygun, O., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8580-8584(2008)
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