|Application ||WB, E|
|Calculated MW||108858 Da|
|Antigen Region||662-691 aa|
|Other Names||ATP-dependent DNA helicase Q5, DNA helicase, RecQ-like type 5, RecQ5, RecQ protein-like 5, RECQL5, RECQ5|
|Target/Specificity||This RECQL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-691 amino acids from the Central region of human RECQL5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RECQL5 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform beta is a DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination.|
|Cellular Location||Isoform Beta: Nucleus, nucleoplasm. Note=Recruited to sites of DNA damage, such as single-strand breaks and inter-strand cross-links, and at stalled replication forks. Isoform Gamma: Cytoplasm|
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Provided below are standard protocols that you may find useful for product applications.
RECQL5 may have an important role in DNA metabolism.
Schwendener, S., et al. J. Biol. Chem. 285(21):15739-15745(2010)
Islam, M.N., et al. Mol. Cell. Biol. 30(10):2460-2472(2010)
Speina, E., et al. Nucleic Acids Res. 38(9):2904-2916(2010)
Aygun, O., et al. J. Biol. Chem. 284(35):23197-23203(2009)
Aygun, O., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8580-8584(2008)
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