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LRRC8A Antibody(C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LRRC8A Antibody(C-term) AP19519b
    All lanes : Anti-LRRC8A Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 94 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8IWT6
Other Accession Q4V8I7, Q80WG5, NP_062540.2
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 94199 Da
Antigen Region 782-810 aa
Additional Information
Gene ID 56262
Other Names Volume-regulated anion channel subunit LRRC8A, Leucine-rich repeat-containing protein 8A, Swelling protein 1, LRRC8A, KIAA1437, LRRC8, SWELL1
Target/Specificity This LRRC8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782-810 amino acids from the C-terminal region of human LRRC8A.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLRRC8A Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LRRC8A
Synonyms KIAA1437, LRRC8, SWELL1
Function Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine (PubMed:24725410, PubMed:24790029, PubMed:26530471, PubMed:26824658). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658). Can form functional channels by itself (in vitro) (PubMed:26824658). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746). Also required for T-cell development (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein. Note=The leucine-rich repeat (LRR) domain is on the cytoplasmic side of the cell membrane
Tissue Location Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells Expressed on T-cells as well as on B-lineage cells
Research Areas
Citations (0)

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Background

This gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

References

Olsen, J.V., et al. Cell 127(3):635-648(2006)
Smits, G., et al. Mol. Immunol. 41(5):561-562(2004)
Kubota, K., et al. FEBS Lett. 564 (1-2), 147-152 (2004) :
Sawada, A., et al. J. Clin. Invest. 112(11):1707-1713(2003)
Conley, M.E. J. Clin. Invest. 112(11):1636-1638(2003)

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$ 295.00
$ 99.00
Cat# AP19519b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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