|Application ||WB, E|
|Calculated MW||117188 Da|
|Antigen Region||51-77 aa|
|Other Names||Probable E3 ubiquitin-protein ligase HERC3, 632-, HECT domain and RCC1-like domain-containing protein 3, HERC3, KIAA0032|
|Target/Specificity||This HERC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human HERC3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HERC3 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.|
|Cellular Location||Cytoplasm. Cytoplasmic vesicle. Note=Also found in vesicular-like structures|
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Provided below are standard protocols that you may find useful for product applications.
HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hochrainer, K., et al. Genomics 85(2):153-164(2005)
Cruz, C., et al. FEBS Lett. 488 (1-2), 74-80 (2001) :
Cruz, C., et al. Cytogenet. Cell Genet. 87 (3-4), 263-264 (1999) :
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