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EIF4E2 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EIF4E2 Antibody AP1955d
    Western blot analysis of EIF4E2 Antibody (Cat. #AP1955d) in NCI-H460 cell line lysates (35ug/lane). EIF4E2 (arrow) was detected using the purified Pab.
  • WB - EIF4E2 Antibody AP1955d
    Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody (Cat. #AP1955d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.
  • IHC-P - EIF4E2 Antibody AP1955d
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60573
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28362 Da
Additional Information
Gene ID 9470
Other Names Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3
Target/Specificity This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4E2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4E2
Synonyms EIF4EL3
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Citations (0)

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EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.


Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998).
Mao, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(14):8175-8180 (1998).
Tee, A.R., et al., FEBS Lett. 564 (1-2), 58-62 (2004) (): ().

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Cat# AP1955d
(40 western blots)
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