|Application ||IHC-P, WB, E|
|Calculated MW||28362 Da|
|Other Names||Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3|
|Target/Specificity||This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF4E2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.|
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Provided below are standard protocols that you may find useful for product applications.
EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998).
Mao, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(14):8175-8180 (1998).
Tee, A.R., et al., FEBS Lett. 564 (1-2), 58-62 (2004) (): ().
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