Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Histones are basic nuclear proteins that are responsible
for the nucleosome structure of the chromosomal fiber in
eukaryotes. Two molecules of each of the four core histones (H2A,
H2B, H3, and H4) form an octamer, around which approximately 146 bp
of DNA is wrapped in repeating units, called nucleosomes. The
linker histone, H1, interacts with linker DNA between nucleosomes
and functions in the compaction of chromatin into higher order
structures. This gene is intronless and encodes a member of the
histone H2B family. Transcripts from this gene lack polyA tails but
instead contain a palindromic termination element. This gene is
found in the histone microcluster on chromosome 6p21.33. [provided
by RefSeq].
References
Shi, J., et al. Nature 460(7256):753-757(2009)
Benyamin, B., et al. Am. J. Hum. Genet. 84(1):60-65(2009)
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Beck, H.C., et al. Mol. Cell Proteomics 5(7):1314-1325(2006)
Pavri, R., et al. Cell 125(4):703-717(2006)