|Application ||WB, IHC-P, E|
|Other Accession||P68037, Q3MHP1, NP_003338.1|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||17862 Da|
|Antigen Region||106-135 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, UBE2L3, UBCE7, UBCH7|
|Target/Specificity||This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the C-terminal region of human UBE2L3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2L3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Ubiquitous, with highest expression in testis.|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Purbeck, C., et al. Biochemistry 49(7):1361-1363(2010)
Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009)
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