|Application ||WB, E|
|Other Accession||Q4R4T8, NP_005597.3|
|Calculated MW||49411 Da|
|Antigen Region||83-112 aa|
|Other Names||Legumain, Asparaginyl endopeptidase, Protease, cysteine 1, LGMN, PRSC1|
|Target/Specificity||This LGMN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the N-terminal region of human LGMN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LGMN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.|
|Tissue Location||Ubiquitous. Particularly abundant in kidney, heart and placenta|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
Clerin, V., et al. Atherosclerosis 201(1):53-66(2008)
Liu, Z., et al. Mol. Cell 29(6):665-678(2008)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Dusso, A.S., et al. Am. J. Physiol. Renal Physiol. 289 (1), F8-F28 (2005) :
Murthy, R.V., et al. Clin. Cancer Res. 11(6):2293-2299(2005)
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