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SF3A2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SF3A2 Antibody (Center) AP20040c
    SF3A2 Antibody (Center) (Cat. #AP20040c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SF3A2 antibody detected the SF3A2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15428
Other Accession Q6AXT8, Q62203, A5PJN8, NP_009096.2
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49256 Da
Antigen Region 166-194 aa
Additional Information
Gene ID 8175
Other Names Splicing factor 3A subunit 2, SF3a66, Spliceosome-associated protein 62, SAP 62, SF3A2, SAP62
Target/Specificity This SF3A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human SF3A2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSF3A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SF3A2
Synonyms SAP62
Function Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00130}
Citations (0)

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This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.


Rose, J. Phd, et al. Mol. Med. (2010) In press :
Tanackovic, G., et al. Mol. Biol. Cell 16(3):1366-1377(2005)
Nesic, D., et al. J. Cell. Sci. 117 (PT 19), 4423-4433 (2004) :
Takenaka, K., et al. Biochem. J. 382 (PT 1), 223-230 (2004) :
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)

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$ 295.00
$ 99.00
Cat# AP20040c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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