- CITATIONS: 2
|Application ||IHC-P, IF, E|
|Calculated MW||177439 Da|
|Antigen Region||1560-1590 aa|
|Other Names||Nestin, NES|
|Target/Specificity||This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560~1590 amino acids from the C-terminal region of human nestin.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Nestin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).|
|Tissue Location||CNS stem cells.|
Provided below are standard protocols that you may find useful for product applications.
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Yaworsky, P.J., et al., Dev. Biol. 205(2):309-321 (1999).
Dahlstrand, J., et al., J. Cell. Sci. 103 (Pt 2), 589-597 (1992).
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