RNASE1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P07998 |
Other Accession | NP_937878.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 17644 Da |
Antigen Region | 14-42 aa |
Gene ID | 6035 |
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Other Names | Ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, Ribonuclease 1, RNase 1, Ribonuclease A, RNase A, RNASE1, RIB1, RNS1 |
Target/Specificity | This RNASE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RNASE1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RNASE1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNASE1 |
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Synonyms | RIB1, RNS1 |
Function | Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double- stranded RNA. |
Cellular Location | Secreted. |
Tissue Location | Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion) |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].
References
D'Alessio, G. Biopolymers 91(12):989-994(2009)
Kover, K.E., et al. J. Mol. Biol. 379(5):953-965(2008)
Ueki, M., et al. Biochem. Genet. 46 (3-4), 145-153 (2008) :
Johnson, R.J., et al. Biochemistry 46(36):10308-10316(2007)
Cybulski, C., et al. Urol. Int. 79(1):44-49(2007)
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