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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RBCK1 Antibody (N-term)   

RBCK1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RBCK1 Antibody (N-term) AP20236a
    RBCK1 Antibody (N-term) (Cat. #AP20236a) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RBCK1 antibody detected the RBCK1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BYM8
Other Accession NP_112506.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57572 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 10616
Other Names RanBP-type and C3HC4-type zinc finger-containing protein 1, 632-, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, HOIL-1, Hepatitis B virus X-associated protein 4, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3, RBCK1, C20orf18, RNF54, UBCE7IP3, XAP3, XAP4
Target/Specificity This RBCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RBCK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRBCK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBCK1
Synonyms C20orf18, RNF54, UBCE7IP3, XAP3, XAP4
Function E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.
Research Areas
Citations (0)

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The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq].


Gustafsson, N., et al. Cancer Res. 70(3):1265-1274(2010)
Yoshimoto, N., et al. FEBS J. 276(21):6375-6385(2009)
Tokunaga, F., et al. Nat. Cell Biol. 11(2):123-132(2009)
Mullenders, J., et al. PLoS ONE 4 (3), E4798 (2009) :
Zhang, M., et al. Cell Res. 18(11):1096-1104(2008)

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$ 295.00
$ 99.00
Cat# AP20236a
(40 western blots)
Availability: In Stock
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