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TRIM6 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TRIM6  Antibody (N-term) AP20469a
    All lanes : Anti-TRIM6 Antibody (N-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TRIM6  Antibody (N-term) AP20469a
    All lanes : Anti-TRIM6 Antibody (N-term) at 1:2000 dilution Lane 1: Ramos whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TRIM6  Antibody (N-term) AP20469a
    TRIM6 Antibody (N-term) (Cat. #AP20469a) western blot analysis in 293,A549,Ramos cell line lysates (35ug/lane).This demonstrates the TRIM6 antibody detected the TRIM6 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9C030
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56400 Da
Antigen Region 26-55 aa
Additional Information
Gene ID 117854
Other Names Tripartite motif-containing protein 6, RING finger protein 89, TRIM6, RNF89
Target/Specificity This TRIM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human TRIM6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIM6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM6
Synonyms RNF89
Function E3 ubiquitin-protein ligase which ubiquitinates MYC and inhibits its transcription activation activity, maintaining the pluripotency of embryonic stem cells (By similarity). Involved in the synthesis of unanchored K48-linked polyubiquitin chains which interact with and activate the serine/threonine kinase IKBKE, leading to phosphorylation of STAT1 and stimulation of an antiviral response (PubMed:24882218).
Cellular Location Cytoplasm
Research Areas
Citations (0)

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References

Reymond A., et al. EMBO J. 20:2140-2151(2001).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Orimo A., et al. Genomics 69:143-149(2000).

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$ 295.00
$ 99.00
Cat# AP20469a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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