|Application ||WB, IF, E|
|Calculated MW||38571 Da|
|Antigen Region||110-141 aa|
|Other Names||POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3|
|Target/Specificity||This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 amino acids from human OCT4 (OCT3).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||OCT4 (OCT3) Antibody (E125) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||OCT3, OCT4, OTF3|
|Function||Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.|
|Cellular Location||Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern|
|Tissue Location||Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Looijenga, L.H., et al., Cancer Res. 63(9):2244-2250 (2003). Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). Nichols, J., et al., Cell 95(3):379-391 (1998). Crouau-Roy, B., et al., Genomics 21(1):241-243 (1994). Takeda, J., et al., Nucleic Acids Res. 20(17):4613-4620 (1992).
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