|Application ||WB, IF, E|
|Calculated MW||52737 Da|
|Antigen Region||45-66 aa|
|Other Names||Periphilin-1, Gastric cancer antigen Ga50, PPHLN1|
|Target/Specificity||This PPHLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-66 amino acids from the N-terminal region of human PPHLN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PPHLN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (Probable).|
|Cellular Location||Nucleus Cytoplasm. Chromosome. Note=In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416).|
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Provided below are standard protocols that you may find useful for product applications.
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Imami K., et al. Anal. Sci. 24:161-166(2008).
Line A., et al. Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., et al. J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).
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