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PPHLN1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - PPHLN1 Antibody (N-term) AP20529a
    Fluorescent image of Hela cells stained with PPHLN1 Antibody (N-term)(Cat#AP20529A). AP20529A was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - PPHLN1 Antibody (N-term) AP20529a
    PPHLN1 Antibody (N-term) (Cat. #AP20529a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PPHLN1 antibody detected the PPHLN1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NEY8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52737 Da
Antigen Region 45-66 aa
Additional Information
Gene ID 51535
Other Names Periphilin-1, Gastric cancer antigen Ga50, PPHLN1
Target/Specificity This PPHLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-66 amino acids from the N-terminal region of human PPHLN1.
Dilution IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPHLN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Cellular Location Nucleus. Cytoplasm. Note=In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.


Imami K., et al. Anal. Sci. 24:161-166(2008).
Line A., et al. Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., et al. J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 295.00
$ 99.00
Cat# AP20529a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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