|Application ||WB, IF, E|
|Calculated MW||52737 Da|
|Antigen Region||45-66 aa|
|Other Names||Periphilin-1, Gastric cancer antigen Ga50, PPHLN1|
|Target/Specificity||This PPHLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-66 amino acids from the N-terminal region of human PPHLN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PPHLN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (Probable).|
|Cellular Location||Nucleus Cytoplasm. Chromosome. Note=In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416).|
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Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Imami K., et al. Anal. Sci. 24:161-166(2008).
Line A., et al. Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., et al. J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).
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