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SNAI1 Antibody (N-term D24)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SNAI1 Antibody (N-term D24) AP2054a
    HepG2 cells were incubated with HGF for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snai1 protein levels and beta actin as loading control were analyzed by WB.
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  • WB - SNAI1 Antibody (N-term D24) AP2054a
    Western blot analysis of SNAI1 (arrow) using rabbit polyclonal hSNAI1-D24 (Cat. #AP2054a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAI1 gene (Lane 2).
    detail
  • IHC-P - SNAI1 Antibody (N-term D24) AP2054a
    Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAI1 antibody (N-term D24) (Cat.#AP2054a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 36
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O95863
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29083 Da
Antigen Region 9-39 aa
Additional Information
Gene ID 6615
Other Names Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna, SNAI1, SNAH
Target/Specificity This SNAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SNAI1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNAI1 Antibody (N-term D24) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNAI1
Synonyms SNAH
Function Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed:20389281, PubMed:20562920). The N- terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed:20389281, PubMed:21300290, PubMed:23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
Cellular Location Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs
Tissue Location Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
Research Areas
Citations ( 0 )

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo.

References

Imai, T., et al., Am. J. Pathol. 163(4):1437-1447 (2003). Yokoyama, K., et al., Int. J. Oncol. 22(4):891-898 (2003). Guaita, S., et al., J. Biol. Chem. 277(42):39209-39216 (2002). Blanco, M.J., et al., Oncogene 21(20):3241-3246 (2002). Okubo, T., et al., Cancer Res. 61(4):1338-1346 (2001).

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$ 365.00
$ 140.00
Cat# AP2054a
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