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STRADA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - STRADA Antibody (C-term) AP20607C
    Immunohistochemical analysis of paraffin-embedded H. duodenum section using STRADA Antibody (C-term)(Cat#AP20607C). AP20607C was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IF - STRADA Antibody (C-term) AP20607C
    Fluorescent image of A549 cells stained with STRADA Antibody (C-term)(Cat#AP20607C). AP20607C was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • FC - STRADA Antibody (C-term) AP20607C
    Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term)(green, Cat#AP20607C) compared to an isotype control of rabbit IgG(blue). AP20607C was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • IHC-P - STRADA Antibody (C-term) AP20607C
    Immunohistochemical analysis of paraffin-embedded H. liver section using STRADA Antibody (C-term)(Cat#AP20607C). AP20607C was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - STRADA Antibody (C-term) AP20607C
    Immunohistochemical analysis of paraffin-embedded M. duodenum section using STRADA Antibody (C-term)(Cat#AP20607C). AP20607C was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - STRADA Antibody (C-term) AP20607C
    Immunohistochemical analysis of paraffin-embedded M. liver section using STRADA Antibody (C-term)(Cat#AP20607C). AP20607C was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - STRADA Antibody (C-term) AP20607C
    Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term)(Cat. #AP20607C). AP20607C was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession Q7RTN6
Other Accession Q3UUJ4, Q4R6X5
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48369 Da
Additional Information
Gene ID 92335
Other Names STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96, STRADA, LYK5 {ECO:0000312|EMBL:AAP422801}, STRAD
Target/Specificity This STRADA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-346 amino acids from the C-terminal region of human STRADA.
Dilution IHC-P~~1:25
IF~~1:25
FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTRADA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STRADA
Synonyms LYK5 {ECO:0000312|EMBL:AAP42280.1}, STRA
Function Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Cellular Location Nucleus. Cytoplasm
Research Areas
Citations ( 0 )

Background

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

References

Scanlan M.J.,et al.Cancer Immun. 1:4-4(2001).
Shan Y.X.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:1045-1049(2006).

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$ 295.00
$ 99.00
Cat# AP20607C
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Quantity:
(40 western blots)
Availability: In Stock
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