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VPS4A Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - VPS4A Antibody (N-term) AP20724a
    Western blot analysis of lysate from Hela cell line, using VPS4A Antibody (N-term)(Cat. #AP20724a). AP20724a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UN37
Other Accession Q793F9, Q8VEJ9
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48898 Da
Additional Information
Gene ID 27183
Other Names Vacuolar protein sorting-associated protein 4A, Protein SKD2, VPS4-1, hVPS4, VPS4A {ECO:0000312|EMBL:AAG014701}
Target/Specificity This VPS4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-77amino acids from the N-terminal region of human VPS4A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS4A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS4A {ECO:0000312|EMBL:AAG01470.1}
Function Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Cellular Location Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Midbody. Note=Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515). Localized in two distinct rings on either side of the Flemming body
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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Background

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.

References

Scheuring S.,et al.J. Mol. Biol. 312:469-480(2001).
Beyer A.,et al.Gene 305:47-59(2003).
Ding J.B.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Patejunas G.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).

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$ 295.00
$ 99.00
Cat# AP20724a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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