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MT-ND1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MT-ND1 Antibody (N-term) AP20750a
    Western blot analysis of lysates from Hela, HepG2 cell line (from left to right), using MT-ND1 Antibody (N-term)(Cat. #AP20750a). AP20750a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P03886
Other Accession O79874, P03887, O78747
Reactivity Human
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35661 Da
Additional Information
Gene ID 4535
Other Names NADH-ubiquinone oxidoreductase chain 1, NADH dehydrogenase subunit 1, MT-ND1, MTND1, NADH1, ND1
Target/Specificity This MT-ND1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 34-68 amino acids from the N-terminal region of human MT-ND1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMT-ND1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MT-ND1
Synonyms MTND1, NADH1, ND1
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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Background

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

References

Anderson S.,et al.Nature 290:457-465(1981).
Horai S.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).
Moilanen J.S.,et al.Mol. Biol. Evol. 20:2132-2142(2003).
Ingman M.,et al.Nature 408:708-713(2000).
Ingman M.,et al.Genome Res. 13:1600-1606(2003).

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$ 295.00
$ 99.00
Cat# AP20750a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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