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CYB5R2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYB5R2 Antibody (N-term) AP20834a
    Western blot analysis of lysates from K562 cell line, mouse liver tissue lysate (from left to right), using CYB5R2 Antibody (N-term)(Cat. #AP20834a). AP20834a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q6BCY4
Other Accession Q6AY12, Q3KNK3
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31458 Da
Additional Information
Gene ID 51700
Other Names NADH-cytochrome b5 reductase 2, b5R2, CYB5R2
Target/Specificity This CYB5R2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-67 amino acids from the N-terminal region of human CYB5R2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R2
Function NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4- disulfophenyl]-2H tetrazolium monosodium salt (WST-1).
Tissue Location Restricted expression.
Research Areas
Citations (0)

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Background

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4- disulfophenyl]-2H tetrazolium monosodium salt (WST-1).

References

Zhu H.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:14742-14747(1999).
Baker M.A.,et al.Biol. Reprod. 73:334-342(2005).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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$ 295.00
$ 99.00
Cat# AP20834a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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