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UBB(Ubiquitin) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - UBB(Ubiquitin) Antibody (N-term) AP20853a
    Western blot analysis of lysates from Hela, HepG2, Jurkat cell line, mouse liver, rat kidney tissue(from left to right), using UBB(Ubiquitin) Antibody (N-term)(Cat. #AP20853a). AP20853a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P0CG47
Other Accession P62975, P62972, P0CG69, P62976, P0CG71, Q63429, P0CG68, P0CG50, P0CG48, P0CH28, P0CG51, P0CG49, P0CG62, P0CG53, P62982, P62983, P62979, P15357, P79781, P62992, P62986, P63053, P62984, P0C273, P62987, P18101, P49632, P63048, P0C276, Q8MKD1, P0CG55
Reactivity Human, Mouse, Rat
Predicted Bovine, C.Elegans, Drosophila, Monkey, Pig, Sheep, Chicken, Horse, Hamster, Xenopus, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25762 Da
Additional Information
Gene ID 7314
Other Names Polyubiquitin-B, Ubiquitin, UBB
Target/Specificity This UBB(Ubiquitin) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57amino acids from the N-terminal region of human UBB(Ubiquitin).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBB(Ubiquitin) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBB
Function Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Cellular Location Ubiquitin: Cytoplasm. Nucleus
Research Areas
Citations (0)

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Background

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

References

Baker R.T.,et al.Nucleic Acids Res. 15:443-463(1987).
Tachikui H.,et al.J. Mol. Evol. 57:737-744(2003).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Schlesinger D.H.,et al.Nature 255:423-424(1975).

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$ 295.00
$ 99.00
Cat# AP20853a
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(40 western blots)
Availability: In Stock
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