|Application ||WB, IF, E|
|Calculated MW||83201 Da|
|Other Names||Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1, TLE1|
|Target/Specificity||This TLE1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 187-221 amino acids from the N-terminal region of human TLE1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TLE1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.|
|Cellular Location||Nucleus. Note=Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components|
|Tissue Location||In all tissues examined, mostly in brain, liver and muscle|
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Provided below are standard protocols that you may find useful for product applications.
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Stifani S.,et al.Nat. Genet. 2:119-127(1992).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Wang J.-C.,et al.J. Biol. Chem. 275:18418-18423(2000).
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