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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CHST12 Antibody (N-term)   

CHST12 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - CHST12 Antibody (N-term) AP21028b
    AP21028b staining CHST12 in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - CHST12 Antibody (N-term) AP21028b
    Anti-CHST12 Antibody (N-term) at 1:2000 dilution + HL-60 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CHST12 Antibody (N-term) AP21028b
    Western blot analysis of lysate from human spleen tissue lysate, using CHST12 Antibody (N-term)(Cat. #AP21028b). AP21028b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NRB3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48414 Da
Additional Information
Gene ID 55501
Other Names Carbohydrate sulfotransferase 12, Chondroitin 4-O-sulfotransferase 2, Chondroitin 4-sulfotransferase 2, C4ST-2, C4ST2, Sulfotransferase Hlo, CHST12
Target/Specificity This CHST12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-106 amino acids from the N-terminal region of human CHST12.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHST12 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST12
Function Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung
Research Areas
Citations (0)

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Background

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.

References

Hiraoka N.,et al.J. Biol. Chem. 275:20188-20196(2000).
Xia G.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.W.,et al.Science 300:767-772(2003).

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$ 295.00
$ 99.00
Cat# AP21028b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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