|Application ||WB, IHC-P, E|
|Calculated MW||99406 Da|
|Antigen Region||716-745 aa|
|Other Names||E3 ubiquitin-protein ligase RNF216, 632-, RING finger protein 216, Triad domain-containing protein 3, Ubiquitin-conjugating enzyme 7-interacting protein 1, Zinc finger protein inhibiting NF-kappa-B, RNF216, TRIAD3, UBCE7IP1, ZIN|
|Target/Specificity||This TRIAD3 (UBCE7IP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 716-745 amino acids from the C-terminal region of human TRIAD3 (UBCE7IP1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRIAD3 (UBCE7IP1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||TRIAD3, UBCE7IP1, ZIN|
|Function||Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down- regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis.|
|Tissue Location||Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
UBCE7IP1 is a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates.
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Chen, D., et al., J. Biol. Chem. 277(18):15985-15991 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
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