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E1 Ubiquitin (UBE1) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2113b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
E1 Ubiquitin (UBE1) Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | P22314 |
| Other Accession | Q5U300, Q29504, Q02053, A3KMV5 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat, Rabbit |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 117849 Da |
E1 Ubiquitin (UBE1) Antibody (C-term) - Additional info | |
| Gene ID 7317 | |
| Other Names UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1 | |
| Target/Specificity This UBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions E1 Ubiquitin (UBE1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
E1 Ubiquitin (UBE1) Antibody (C-term) - Protein Information | |
| Name UBA1 | |
| Synonyms A1S9T, UBE1 | |
| Function Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP | |
E1 Ubiquitin (UBE1) Antibody (C-term) - Related products
AP14555a: UBA1 Antibody (N-term)
AP2113b: E1 Ubiquitin (UBE1) Antibody (C-term)
AP2113c: E1 Ubiquitin (UBE1) Antibody (Center)
E1 Ubiquitin (UBE1) Antibody (C-term) - Application data
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Western blot analysis of E1 Ubiquitin (UBE1) Antibody (C-term) (Cat. #AP2113b) in mouse stomach tissue lysates (35ug/lane). UBE1 (arrow) was detected using the purified Pab.
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The anti-UBE1 Pab (Cat. #AP2113b) is used in Western blot to detect UBE1 in HL-60 cell lysate.
E1 Ubiquitin (UBE1) Antibody (C-term) - Research Areas
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BACKGROUND
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.
REFERENCES
Ayusawa, D., et al., Cell Struct. Funct. 17(2):113-122 (1992). Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88(1):258-262 (1991). Kudo, M., et al., Exp. Cell Res. 192(1):110-117 (1991). Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53(1):20-22 (1990). Zacksenhaus, E., et al., EMBO J. 9(9):2923-2929 (1990).
