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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   E1 Ubiquitin (UBE1) Antibody (C-term)   

E1 Ubiquitin (UBE1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - E1 Ubiquitin (UBE1) Antibody (C-term) AP2113b
    Western blot analysis of E1 Ubiquitin (UBE1) Antibody (C-term) (Cat. #AP2113b) in mouse stomach tissue lysates (35ug/lane). UBE1 (arrow) was detected using the purified Pab.
  • WB - E1 Ubiquitin (UBE1) Antibody (C-term) AP2113b
    The anti-UBE1 Pab (Cat. #AP2113b) is used in Western blot to detect UBE1 in HL-60 cell lysate.
  • IHC-P - E1 Ubiquitin (UBE1) Antibody (C-term) AP2113b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - E1 Ubiquitin (UBE1) Antibody (C-term) AP2113b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody (C-term) (Cat.#AP2113b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P22314
Other Accession Q5U300, Q29504, Q02053, A3KMV5
Reactivity Human, Mouse
Predicted Bovine, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 117849 Da
Antigen Region 1026-1058 aa
Additional Information
Gene ID 7317
Other Names Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1
Target/Specificity This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE1 Ubiquitin (UBE1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBA1
Synonyms A1S9T, UBE1
Function Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system. Activates ubiquitin by first adenylating its C- terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
Cellular Location Cytoplasm. Mitochondrion. Nucleus. Isoform 2: Cytoplasm.
Research Areas
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Background

UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.

References

Ayusawa, D., et al., Cell Struct. Funct. 17(2):113-122 (1992). Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88(1):258-262 (1991). Kudo, M., et al., Exp. Cell Res. 192(1):110-117 (1991). Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53(1):20-22 (1990). Zacksenhaus, E., et al., EMBO J. 9(9):2923-2929 (1990).

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$ 295.00
$ 99.00
Cat# AP2113b
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(40 western blots)
Availability: In Stock
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