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Mouse Noto Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Noto Antibody (C-term) AP21141a
    Western blot analysis of lysates from mouse liver, mouse pancreas tissue lysate (from left to right), using Noto Antibody (C-term)(Cat. #AP21141a). AP21141a was diluted at 1:4000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - Mouse Noto Antibody (C-term) AP21141a
    Western blot analysis of lysate from mouse liver tissue lysate, using Noto Antibody (C-term)(Cat. #AP21141a). AP21141a was diluted at 1:2000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q5TIS6
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 26322 Da
Additional Information
Gene ID 384452
Other Names Homeobox protein notochord, Noto, Not
Target/Specificity This Mouse Noto antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 200-234 amino acids from the C-terminal region of Mouse Noto.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Noto Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Noto
Synonyms Not
Function Transcription regulator acting downstream of both FOXA2 and T during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Research Areas
Citations (0)

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Background

Transcription regulator acting downstream of both FOXA2 and T during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate.

References

Ben Abdelkhalek H.,et al.Genes Dev. 18:1725-1736(2004).
Carninci P.,et al.Science 309:1559-1563(2005).
Yamanaka Y.,et al.Dev. Cell 13:884-896(2007).
Beckers A.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:15765-15770(2007).

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$ 295.00
$ 99.00
Cat# AP21141a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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