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UBE2B Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - UBE2B Antibody (C-term) AP2115b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - UBE2B Antibody (C-term) AP2115b
    Western blot analysis of UBE2B Antibody (C-term) (Cat. #AP2115b) in Ramos cell line lysates (35ug/lane). UBE2B (arrow) was detected using the purified Pab.
  • WB - UBE2B Antibody (C-term) AP2115b
    Western blot analysis of UBE2B (arrow) using rabbit polyclonal UBE2B Antibody (E132) (Cat. #AP2115b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2B gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P63146
Other Accession P63149, P63148, P63147, Q32P99, Q9Z255, P49459, NP_003328
Reactivity Human
Predicted Mouse, Bovine, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 17312 Da
Antigen Region 117-146 aa
Additional Information
Gene ID 7320
Other Names Ubiquitin-conjugating enzyme E2 B, RAD6 homolog B, HR6B, hHR6B, Ubiquitin carrier protein B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B, UBE2B, RAD6B
Target/Specificity This UBE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human UBE2B.
Dilution IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2B
Synonyms RAD6B
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys- 164'. May be involved in neurite outgrowth.
Cellular Location Cell membrane. Nucleus. Note=In peripheral neurons, expressed both at the plasma membrane and in nuclei.
Research Areas
Citations (0)

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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.


Koken, M.H., et al., Genomics 12(3):447-453 (1992).
Koken, M.H., et al., Proc. Natl. Acad. Sci. U.S.A. 88(20):8865-8869 (1991).
Schneider, R., et al., EMBO J. 9(5):1431-1435 (1990).

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$ 295.00
$ 99.00
Cat# AP2115b
(40 western blots)
Availability: In Stock
Bulk Size
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