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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Mouse Nkx2-5 Antibody (Center)   

Mouse Nkx2-5 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - Mouse Nkx2-5 Antibody (Center) AP21222c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP21222c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • IHC-P - Mouse Nkx2-5 Antibody (Center) AP21222c
    AP21222c staining Mouse Nkx2-5 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - Mouse Nkx2-5 Antibody (Center) AP21222c
    All lanes : Anti-Nkx2-5 Antibody (Center) at 1:2000 dilution Lane 1: mouse stomach lysates Lane 2: mouse spleen lysates Lane 3: mouse heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Mouse Nkx2-5 Antibody (Center) AP21222c
    All lanes : Anti-Nkx2-5 Antibody (Center) at 1:1000 dilution Lane 1: mouse spleen lysates Lane 2: mouse stomach lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, IHC-P, WB, E
Primary Accession P42582
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 34163 Da
Additional Information
Gene ID 18091
Other Names Homeobox protein Nkx-25, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, Nkx2-5, Csx, Nkx-25, Nkx2e
Target/Specificity This mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 98-133 amino acids from the Central region of mouse Nkx2-5.
Dilution IF~~1:25
IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Nkx2-5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nkx2-5
Synonyms Csx, Nkx-2.5, Nkx2e
Function Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.
Cellular Location Nucleus.
Tissue Location Predominantly in the adult and embryonic heart, and to a lesser extent in lingual muscle, spleen and stomach
Research Areas
Citations (0)

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Background

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

References

Lints T.J.,et al.Development 119:419-431(1993).
Lints T.J.,et al.Development 119:969-969(1993).
Searcy R.D.,et al.Development 125:4461-4470(1998).
Komuro I.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8145-8149(1993).
Kim Y.H.,et al.J. Biol. Chem. 273:25875-25879(1998).

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$ 295.00
$ 99.00
Cat# AP21222c
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Quantity:
(40 western blots)
Availability: In Stock
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