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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP13 Antibody (Center)   

USP13 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - USP13 Antibody (Center) AP2141c
    Anti-USP13 Antibody (V404)at 1:2000 dilution + human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 97. 3 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - USP13 Antibody (Center) AP2141c
    The anti-USP13 Pab (Cat. #AP2141c) is used in Western blot to detect USP13 in HeLa cell lysate.
  • WB - USP13 Antibody (Center) AP2141c
    Detection of USP13 in HeLa cells expressing exogenous USP13 by anti-USP13 Pab (Cat. #AP2141c). (Data provided by Amy Chen, Burnham Institute for Medical Research)
  • IHC-P - USP13 Antibody (Center) AP2141c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92995
Other Accession Q5BKP2, E1BMF7, NP_003931
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 97327 Da
Antigen Region 389-419 aa
Additional Information
Gene ID 8975
Other Names Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, ISOT-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, USP13, ISOT3
Target/Specificity This USP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-419 amino acids from the Central region of human USP13.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP13 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP13
Synonyms ISOT3
Function Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Tissue Location Highly expressed in ovary and testes.
Research Areas
Citations (0)

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Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.


Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).
Timms, K.M., et al., Gene 217 (1-2), 101-106 (1998).

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$ 295.00
$ 99.00
Cat# AP2141c
(40 western blots)
Availability: In Stock
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