|Application ||WB, E|
|Calculated MW||120548 Da|
|Other Names||Rap guanine nucleotide exchange factor 1, CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G, RAPGEF1, GRF2|
|Target/Specificity||This RAPGEF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 536-569 amino acids from the Central region of human RAPGEF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAPGEF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.|
|Cellular Location||Early endosome|
|Tissue Location||Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver|
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Provided below are standard protocols that you may find useful for product applications.
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Tanaka S.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994).
Knudsen B.,et al.J. Biol. Chem. 269:32781-32787(1994).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Humphray S.J.,et al.Nature 429:369-374(2004).
Matsuda M.,et al.J. Biol. Chem. 271:14468-14472(1996).
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