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(Mouse) Mlf1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - (Mouse) Mlf1 Antibody (Center) AP21445c
    Anti-Mlf1 Antibody (Center)at 1:500 dilution + mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9QWV4
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 30432 Da
Additional Information
Gene ID 17349
Other Names Myeloid leukemia factor 1, Hematopoietic lineage switch 7, Myelodysplasia-myeloid leukemia factor 1, Mlf1, Hls7
Target/Specificity This Mouse Mlf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-126 amino acids from the Central region of Mouse Mlf1.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Mlf1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mlf1
Synonyms Hls7
Function Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Cellular Location Cytoplasm. Nucleus. Note=In non- hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus
Tissue Location Highly expressed in skeletal muscle, heart, testis. Also found in lung, but not in spleen, thymus, bone marrow, liver and kidney.
Research Areas
Citations (0)

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Background

Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

References

Hitzler J.K.,et al.Am. J. Pathol. 155:53-59(1999).
Williams J.H.,et al.EMBO J. 18:5559-5566(1999).
Lim R.,et al.J. Biol. Chem. 277:40997-41008(2002).
Winteringham L.N.,et al.Oncogene 23:5105-5109(2004).
Winteringham L.N.,et al.J. Biol. Chem. 281:38791-38800(2006).

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$ 295.00
$ 99.00
Cat# AP21445c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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