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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP22 Antibody (C-term)   

USP22 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - USP22 Antibody (C-term) AP2148b
    Western blot analysis of lysates from HepG2, A549 cell line (from left to right), using USP22 Antibody (C-term)(Cat. #AP2148B). AP2148B was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - USP22 Antibody (C-term) AP2148b
    Western blot analysis of USP22 Antibody (C-term) (Cat. #AP2148b) in Jurkat cell line lysates (35ug/lane). USP22 (arrow) was detected using the purified Pab.
  • IHC-P - USP22 Antibody (C-term) AP2148b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UPT9
Other Accession Q8CEG8, A6NNY8, Q5DU02, A6H8I0, P0C8Z3, Q6DCJ1, Q6GNI6
Reactivity Human
Predicted Mouse, Zebrafish, Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59961 Da
Antigen Region 351-380 aa
Additional Information
Gene ID 23326
Other Names Ubiquitin carboxyl-terminal hydrolase 22, Deubiquitinating enzyme 22, Ubiquitin thioesterase 22, Ubiquitin-specific-processing protease 22, USP22, KIAA1063, USP3L
Target/Specificity This USP22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the C-terminal region of human USP22.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP22 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP22
Synonyms KIAA1063, USP3L
Function Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
Cellular Location Nucleus.
Tissue Location Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
Research Areas
Citations ( 0 )


Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.


Kikuno, R., et al., DNA Res. 6(3):197-205 (1999).

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$ 295.00
$ 99.00
Cat# AP2148b
(40 western blots)
Availability: In Stock
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