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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   AMFR Antibody (N-term)   

AMFR Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AMFR Antibody (N-term) AP2162a
    Western blot analysis of AMFR (arrow) using AMFR Antibody (N-term) (Cat.#AP2162a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).
  • IHC - AMFR Antibody (N-term) AP2162a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC - AMFR Antibody (N-term) AP2162a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term) (Cat.#AP2162a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - AMFR Antibody (N-term) AP2162a
    Flow cytometric analysis of HepG2 cells using AMFR Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
ApplicationWB, IHC, FC, E
Primary AccessionQ9UKV5
Other AccessionP26442
ReactivityHuman
HostRabbit
ClonalityPolyclonal
IsotypeRabbit Ig
Calculated MW72996 Da
Antigen Region571-601 aa
Additional Information
Gene ID267
Other NamesE3 ubiquitin-protein ligase AMFR, 632-, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45
Target/SpecificityThis AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-601 amino acids from the N-terminal region of human AMFR.
DilutionWB~~1:1000
IHC~~1:50~100
FC~~1:10~50
FormatPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Protein Information
NameAMFR
SynonymsRNF45
Function E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas
Citations ( 0 )

Background

Autocrine motility factor (AMF) is a protein secreted by tumor cells that stimulates tumor motility. The gene for AMFR encodes a 323-amino acid polypeptide that has a single transmembrane domain and several putative glycosylation sites. The protein sequence has some homology to human tumor protein p53.

References

Huang, B., et al., Biochem. Biophys. Res. Commun. 212(3):727-742 (1995).
Watanabe, H., et al., J. Biol. Chem. 266(20):13442-13448 (1991).

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$ 265.00
$ 95.00
Cat# AP2162a
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(40 western blots)
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