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AMFR Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # SizeAvailability Available Date  
AP2162a 400 µl
(40 western blots)
Backorder 4 months
AP2162a-ev 80 µl
(8 western blots)
Backorder 4 months
  • Specification
  • Citations : 1
  • Reviews
  • Protocols
  • Background

AMFR Antibody (N-term) - Product info

Application
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC
Primary AccessionQ9UKV5
Other AccessionP26442
ReactivityHuman
IsotypeRabbit Ig
Calculated MW72996 Da

AMFR Antibody (N-term) - Additional info

Gene ID 267
Other Names
AMFR; RNF45; E3 ubiquitin-protein ligase AMFR; Autocrine motility factor receptor, isoform 2; RING finger protein 45; gp78
Target/Specificity
This AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-601 amino acids from the N-terminal region of human AMFR.
Dilution
WB~~1:1000
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
AMFR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

AMFR Antibody (N-term) - Protein Information

Name AMFR
Synonyms RNF45
Function
E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
Cellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein

AMFR Antibody (N-term) - Related products

AP14958c: AMFR Antibody (Center)

BP14958c: AMFR Antibody (Center) Blocking Peptide

BP2162a: AMFR Antibody (N-term) Blocking Peptide

AMFR Antibody (N-term) - Research Areas

Application data

  • WB - AMFR Antibody (N-term) AP2162a

    Western blot analysis of AMFR (arrow) using AMFR Antibody (N-term) (Cat.#AP2162a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).

  • IHC - AMFR Antibody (N-term) AP2162a

    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

  • IHC - AMFR Antibody (N-term) AP2162a

    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term) (Cat.#AP2162a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • FC - AMFR Antibody (N-term) AP2162a

    Flow cytometric analysis of HepG2 cells using AMFR Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • WB - AMFR Antibody (N-term) AP2162a
  • IHC - AMFR Antibody (N-term) AP2162a
  • IHC - AMFR Antibody (N-term) AP2162a
  • FC - AMFR Antibody (N-term) AP2162a

ELITE™ Custom Antibody Services

  • Phosphoglucose isomerase/autocrine motility factor promotes melanoma cell migration through ERK activation dependent on autocrine production of interleukin-8. Author : Araki K, Shimura T, Yajima T, Tsutsumi S, Suzuki H, Okada K, Kobayashi T, Raz A, Kuwano H.
    J Biol Chem. 2009 Nov 20;284(47):32305-11. doi: 10.1074/jbc.M109.008250. Epub 2009 Sep 30.
    PubMed® Id : 19801670

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Autocrine motility factor (AMF) is a protein secreted by tumor cells that stimulates tumor motility. The gene for AMFR encodes a 323-amino acid polypeptide that has a single transmembrane domain and several putative glycosylation sites. The protein sequence has some homology to human tumor protein p53.

REFERENCES

Huang, B., et al., Biochem. Biophys. Res. Commun. 212(3):727-742 (1995).
Watanabe, H., et al., J. Biol. Chem. 266(20):13442-13448 (1991).