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TST Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - TST Antibody (Center) AP21670c
    AP21670c staining TST in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - TST Antibody (Center) AP21670c
    All lanes : Anti-TST Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: rat kidney lysate Lane 4: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q16762
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 33429 Da
Additional Information
Gene ID 7263
Other Names Thiosulfate sulfurtransferase, Rhodanese, TST
Target/Specificity This TST antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 170-203 amino acids from the Central region of human TST.
Dilution IHC-P~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTST Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TST
Function Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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Background

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.

References

Aita N.,et al.Biochem. Biophys. Res. Commun. 231:56-60(1997).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham I.,et al.Nature 402:489-495(1999).

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$ 295.00
$ 99.00
Cat# AP21670c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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