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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RASGRP2 Antibody (N-term)   

RASGRP2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RASGRP2 Antibody (N-term) AP21681a
    Anti-RASGRP2 Antibody (N-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7LDG7
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 69248 Da
Additional Information
Gene ID 10235
Other Names RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7
Target/Specificity This RASGRP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-142 amino acids from the N-terminal region of human RASGRP2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRASGRP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RASGRP2
Synonyms CDC25L, MCG7
Function Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
Cellular Location Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity)
Tissue Location Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
Research Areas
Citations (0)

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Background

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.

References

Kedra D.,et al.Hum. Genet. 100:611-619(1997).
Kawasaki H.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:13278-13283(1998).
Clyde-Smith J.,et al.J. Biol. Chem. 275:32260-32267(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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Cat# AP21681a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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