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NDUFA1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - NDUFA1 Antibody (Center) AP21705c
    All lanes : Anti-NDUFA1 Antibody (Center) at 1:2000 dilution Lane 1: A-673 whole cell lysate Lane 2: human skeletal muscle lysate Lane 3: mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O15239
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 8072 Da
Additional Information
Gene ID 4694
Other Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, Complex I-MWFE, CI-MWFE, NADH-ubiquinone oxidoreductase MWFE subunit, NDUFA1
Target/Specificity This NDUFA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-59 amino acids from the Central region of human NDUFA1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFA1
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Matrix side
Tissue Location Primarily expressed in heart and skeletal muscle
Research Areas
Citations (0)

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Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

References

Zhuchenko O.,et al.Genomics 37:281-288(1996).
Frattini A.,et al.Gene 192:291-298(1997).
Zhuchenko O.P.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Murray J.,et al.J. Biol. Chem. 278:13619-13622(2003).
Sjoeblom T.,et al.Science 314:268-274(2006).

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$ 295.00
$ 99.00
Cat# AP21705c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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