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TMEM97 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TMEM97 Antibody (N-term) AP21745a
    Anti-TMEM97 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TMEM97 Antibody (N-term) AP21745a
    All lanes : Anti-TMEM97 Antibody (N-term) at 1:8000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q5BJF2
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 20848 Da
Additional Information
Gene ID 27346
Other Names Transmembrane protein 97, Protein MAC30, TMEM97, MAC30
Target/Specificity This TMEM97 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from the N-terminal region of human TMEM97.
Dilution WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTMEM97 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TMEM97
Synonyms MAC30
Function Plays a role as a regulator of cellular cholesterol homeostasis (PubMed:19583955). May function as sterol isomerase (PubMed:25566323).
Cellular Location Nucleus membrane. Rough endoplasmic reticulum. Cell membrane. Lysosome. Membrane; Multi-pass membrane protein. Note=Localized in sterol-depleted cells at cell membrane and in lysosomes.
Tissue Location Widely expressed in normal tissues. Expressed in pancreatic, renal, breast, colon, ovarian surface epithelial (OSE) cells and meningioma cancers. Expressed in ovarian cancer; expression is reduced relative to OSE cells
Research Areas
Citations (0)

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Background

Plays a role as a regulator of cellular cholesterol homeostasis.

References

Murphy M.,et al.Cell Growth Differ. 4:715-722(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kayed H.,et al.Histol. Histopathol. 19:1021-1031(2004).
Wilcox C.B.,et al.BMC Cancer 7:223-223(2007).
Bartz F.,et al.Cell Metab. 10:63-75(2009).

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$ 99.00
Cat# AP21745a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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