|Application ||WB, IHC-P, E|
|Calculated MW||59180 Da|
|Antigen Region||326-356 aa|
|Other Names||Signal transducing adapter molecule 1, STAM-1, STAM, STAM1|
|Target/Specificity||This STAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-356 amino acids from the C-terminal region of human STAM.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||STAM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.|
|Cellular Location||Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). STAM for 'signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540-amino acid protein precipitated by anti-phosphotyrosine. Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types. The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM). It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.
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